Abstract
Light harvesting peripheral complexes (LH2) and carotenoids free-reaction centres were isolated from purple photosynthetic bacteria Rhodospirillum molischianum and Rhodobacter sphaeroides, respectively. The LH2 complexes without and with various amounts of reaction centres were immobilized in polyacrylamide gel. The absorption and photoacoustic spectra of such samples were measured. The changes due to continuous light illumination in absorption and photoacoustic spectra were investigated. The changes in absorption generated by flash were also measured. The conclusions concerning the photoreactions in antenna and reaction centre chromophores, the storage of energy in reaction centres as well as the protection of LH2 chromophores against overexcitation by heat emission and excitation transfer to reaction centres are discussed.
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