Abstract
The methanolic leaf extract (MLE) of medicinally important plant Holoptelea integrifolia (Roxb.) Planch, a tree member of Ulmaceae family, exhibits prominent photoprotective and antioxidant activity mainly due to presence of phenols, flavonoids and tannins in notable amounts. Photoprotective efficacy (Sun Protection Factor value) of MLE was found to be 16.265 ± 0.0077, which can effectively block around 93% UV-B radiation. The transmission erythema (%Te) and transmission pigmentation (%Tp) values are lowest (2.4102 ± 0.620 and 3.050984 ± 0.183 respectively) at highest concentration of MLE (500 μg/ml). The antioxidant potential of MLE was evaluated by Reducing Power Assay (IC50 value: 88.52 ± 0.583 μg/ml), DPPH (IC50 value: 61.96 ± 0.721 μg/ml) and ABTS scavenging (IC50 value: 210.15 ± 0.329 μg/ml) activities. Ultraviolet (UV) ray induced DNA damage assay also revealed that MLE provide ample protection. Among the separated TLC fractions of MLE, four fractions (F2, F3, F4 & F7) were selected for further analysis. Six phenolic acids namely-tannic acid, gallic acid, hydroxybenzoic acid, p-coumaric acid, trans-cinnamic acid, caffeic acid and quercetin were identified from the RP-HPLC chromatograms. Subsequent quantification of these identified phenolics revealed tannic acid as a dominant compound in F2 (45.06 ± 0.241 μg/mg) and F7 (47.46 ± 0.189 μg/mg), along with p-coumaric acid (104.12 ± 0.226 μg/mg) and trans-cinnamic acid (108.19 ± 0.316 μg/mg) in F3 and F4 respectively. So above findings indicate that MLE is endowed with rich source of medicinally potential phytometabolites with prominent UV-blocking and antioxidant property, which offer immense pharmaceutical prospects.
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