Abstract
Abstract— The progressive reduction in temperature of hydro‐alcoholic mixtures is a way of associating aromatic molecules. It is possible to test the solute association, both by absorption and fluorescence measurements, of ‘polymerizable dyes' and solutes of biological interest. It is also possible to test the solute association by electron spin resonance of stable radicals (Wür‐ster's blue). The application of this technique to the study of photoprocesses was performed with flavin mononucleotide (FMN) by electron spin resonance and absorption spectroscopy in order to analyze the mechanism of photoreduction, and the stability of photo‐induced free radicals.The procedure of associating molecules by temperature effect in these solvents can be applied to the spectroscopic studies of the triplet state of aromatics in glassy solutions at 77°K.
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