Photophysical Properties and Rotational Relaxation Dynamics of Neutral Red Bound to β-Cyclodextrin

Abstract

Photophysical properties and the rotational relaxation dynamics of a phenazine based dye, neutral red, have been investigated in aqueous solutions in the presence of β-cyclodextrin (β-CD) using ground-state absorption and steady-state and time-resolved fluorescence measurements. It has been observed that while the neutral form (NR) of the dye forms an inclusion complex with β-CD, its protonated form (NRH+) does not. In the presence of β-CD (10 mM), the pKa value of the dye is estimated to be about 6.06 ± 0.05, much lower than the pKa value of 6.81 ± 0.05 measured in aqueous solution. Both photophysical properties and the rotational relaxation dynamics of NR undergo substantial changes in the presence of β-CD. Steady-state and time-resolved fluorescence studies suggest simultaneous formation of 1:1 and 1:2 complexes of NR/β-CD in aqueous solutions. Time-resolved fluorescence anisotropy measurements confirm that only NR forms an inclusion complex with β-CD and the NR/β-CD complex rotates as a whole with a r...