Abstract
A photonic crystal surface mode imaging (PCSMi) technique is implemented for the simultaneous detection of antibody binding with specific antigens in arrays containing 96- and 384-spots. Like the surface plasmon resonance imaging (SPRi) technique, the presented approach is label-free and permits interrogating an analyte by hundreds of different ligands immobilized in small spots. The adsorption kinetics is recorded with a sub-picogram resolution at every spot simultaneously. Possible implementations of this technique for multiplexed and high-throughput biosensing are discussed.
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