PHOTOMORPHOGENIC RESPONSES TO UV RADIATION III: A COMPARATIVE STUDY OF UVB EFFECTS ON ANTHOCYANIN and FLAVONOID ACCUMULATION IN WILD‐TYPE and aurea MUTANT OF TOMATO (Lycopersicon esculentum MILL.)

Abstract

AbstractThe UV‐mediated induction of anthocyanin and UV‐absorbing compounds was characterized in etiolated hypocotyls of wild‐type and aurea (au) mutant tomato seedlings. Ultraviolet radiation induced significant increases of anthocyanin and UV‐absorbing compounds in hypocotyls of die au mutant and of its isogenic wild‐type, but the differences in the time courses of UV‐induced pigment accumulation indicate mat different photoregulatory mechanisms are involved for each of these two groups of pigments. It appears mat prolonged presence of adequate levels of UVB (290–320nm) energy and consequently the action of a specific UVB photoreceptor are indispensable for the photoinduction of anthocyanin accumulation in UV‐irradiated hypocotyl of the au mutant that is missing the labile phytochrome pool. The large difference found between the wild‐type and the au mutant strongly indicate the involvement of labile phytochrome as the primary functional photoreceptor for the photoinduction of anthocyanin accumulation in wild‐type tomato hypocotyls. The UVB photoreceptor could at least partly replace the action of labile phytochrome (as far as anthocyanin accumulation is concerned) when the functional phytochrome pool is missing as in the au mutant. The general picture of UV‐mediated induction of total UV‐absorbing compounds shows only a macroscopic difference between wild‐type and die au mutant of tomato: the higher initial level (in darkness) of these compounds in die wild‐type in contrast to the au mutant. Although there is UV‐induced accumulation of UV‐absorbing compounds in bom genotypes, the levels in the au mutant never reach mat of the wild‐type under the same UV exposure. A UVB photosensor may play a more important role in the photoinduction of UV‐absorbing compounds. Indeed, in the absence of labile phytochrome, i.e. in the au mutant, a UVB‐absorbing photoreceptor alone is able to establish high responsiveness for the UV‐induced flavonoid accumulation.