Photometrische Titration der Särkepolysaccharide mit Jod. II. Mitt. Hydrolyse mechano‐chemisch abgebauter Amylose durch β‐Amylase

Abstract

AbstractPhotometric Titration of Starch Polysaccharides by Iodine. Part 2: Hydrolysis of Mechano Chemically Degraded Amylose by β‐Amylase. With photometric titration of amylose by means of iodine, the course of the graph depends on molecular weight distribution, average molecular size and concentration of polysaccharides. Respective data are: size of the linear part of the titration graph, total iodine concentration at half saturation of amylose by iodine, and maximum value of extinction. This new method is applied to examine β‐amylolysis of natural amylose of various molecular size. High‐molecular substrates are degraded according to a single‐chain mechanism and amyloses having been swing‐milled for more than 48 h are degraded by a multichain mechanism. The change in the effect of β‐amylase occurs in the range between Pn 130 and 200, This change is to be contributed to differences in the molecular configuration of the substrate.