Abstract
The use of acetonitrile—water mobile phases for reversed-phase high-performance liquid chromatography allows photometric detection at wavelengths as low as 185 nm in both the isocratic and gradient elution modes. At 185 nm almost all compound types exhibit usable detection sensitivity, making the UV spectrophotometer a “universal” detector. Application of detection at 185 nm for the gradient elution separation of various polyethylene glycols allows the routine analysis of mixtures of these compounds, either in unesterified samples or in samples containing both the unesterified and esterified derivatives.
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