Abstract
A simple and sensitive method for the assay of hepatic microsomal epoxide hydrolase activity by using safrole oxide (SAFO) as the substrate is described. It involves differential extraction of the unreacted substrate with n-hexane from the incubation medium containing the reaction product, safrole glycol (SAFG) followed by measurement of absorbancy at 288 nm of the separated hexane layer. A stoichiometric relationship between the SAFO disappearance and SAFG formation was confirmed in the enzymatic reaction.
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