Abstract

Staphylococcus aureus is most common causes of hospital-acquired infections and food-associated disease. In the last years, sensing platform based on fluorescence- nanomaterials and nanocomposites have been attracted a great attention for detection of bacteria and macromolecules. In this study, a new modified mesoporous silica SBA-15 with ZnO and Au nanoparticles (SBA-15@ZnO/Au) was prepared to enhance the photoluminescence emission of SBA-15 matrix and investigate the potential use as a nano probe for detection of S. aureus bacteria. Au nanoparticles were added to the SBA-15 after loading ZnO nanoparticles and the SBA-15@ZnO/Au nanocomposite was successfully fabricated. The Structural, morphological, physicochemical and optical properties of the prepared samples have been studied. The low-angle X-ray diffraction (XRD) pattern showed highly ordered two-dimensional hexagonal lattice of SBA-15 and the wide-angle XRD pattern proved the formation of ZnO and Au nanoparticles. The results of the Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) analysis showed that the prepared nanocomposite have a regular two dimensional hexagonal structure with cylindrical channels even after loading ZnO and Au nanoparticles. According to Nitrogen adsorption–desorption isotherm measurement, the pore diameter and the specific surface area of SBA-15@ZnO/Au nanocomposite have been obtained 3.15 nm and 604.19 m2/g. Photoluminescence (PL) spectra of bare SBA-15@ZnO/Au nanocomposite showed three peaks at central wavelength of 410, 500 and 750 nm arising from electron transfer process and radiative decay of collective plasmonic states. After addition of S. aureus bacteria, the nano probe-target complex was formed and the PL re-emission at 640 nm and quenching of PL emissions at 750 and 410 nm was observed. Furthermore, the new nanocomposite was demonstrated to have the proper sensitivity toward bacteria concentrations. The results can open promising prospect for this nanocomposite as a nano probe for detection of S. aureus in the UV–Visible region of electromagnetic spectrum.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call