Abstract
The use of low-dimensional inorganic or organic nanomaterials has advantages for DNA and protein recognition due to their sensitivity, accuracy, and physical size matching. In this research, poly(3-methylthiophene) (P3MT) nanowires (NWs) are electrochemically prepared with dopant followed by functionalization with probe DNA (pDNA) sequence through electrostatic interaction. Various lengths of pDNA sequences (10-, 20- and 30-mer) are conjugated to the P3MT NWs respectively followed with hybridization with their complementary target DNA (tDNA) sequences. The nanoscale photoluminescence (PL) properties of the P3MT NWs are studied throughout the whole process at solid state. In addition, the correlation between the PL enhancement and the double helix DNA with various lengths is demonstrated.
Highlights
The effective conjugation of biological materials with functional condensed matters plays an important role in biological recognition in terms of accuracy, sensitivity, and signal response time [1,2,3,4,5].Organic π-conjugated materials such as polythiophene and polydiacetylene have been considered as active systems for biological recognition due to their compatibility with biological molecules and their properties of optical and electrical signal [6,7,8,9,10,11]
The formation of the P3MT NWs was visualized by transmission electron microscope (TEM)
NWs are conjugated with variousoflengths of probe DNA (pDNA) by hybridization with
Summary
The effective conjugation of biological materials with functional condensed matters plays an important role in biological recognition in terms of accuracy, sensitivity, and signal response time [1,2,3,4,5].Organic π-conjugated materials such as polythiophene and polydiacetylene have been considered as active systems for biological recognition due to their compatibility with biological molecules and their properties of optical and electrical signal [6,7,8,9,10,11]. The effective conjugation of biological materials with functional condensed matters plays an important role in biological recognition in terms of accuracy, sensitivity, and signal response time [1,2,3,4,5]. A simple and effective method of optically-direct (i.e., without fluorescent dye) DNA detection can be proposed by the light-emitting polymer nanowires (NWs) with lightly-doped states [12]. These polymers possess negative counter-ions with dipole characteristics from dopants placed near (much less than 10 nm) the cationic polymer chains [13]. Raman signal enhancement of the NW was achieved due to the resonance effect between the wavelength of the Polymers 2018, 10, 100; doi:10.3390/polym10010100 www.mdpi.com/journal/polymers
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