Abstract

The photoinduced carotenoid synthesis of a Mycobacterium sp. has been studied and found to closely resemble the same phenomenon that has been reported to occur in Neurospora crassa. It has been possible to divide photoinduced carotenogenesis of this Mycobacterium sp. into three distinct phases. 1. 1. The photoinduction step which requires oxygen and light (the blue portion of the visible spectrum). This phase is temperature independent and the only one for which light is necessary. 2. 2. The protein synthesis phase which lasts for the first 2 h of incubation following light stimulation. Chloramphenicol is an effective inhibitor of this phase. 3. 3. The final stage, carotenoid synthesis, is dependent upon the enzymes synthesized in the second step and begins after 1-1.5 h of incubation and lasts approx. 12 h.

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