Abstract
The interaction between colloidal TiO 2 nanoparticles and lysozyme (LYSO) was studied using absorption, steady state and time resolved fluorescence, FT-IR and synchronous fluorescence spectroscopic measurements. The apparent association constant has been deduced from the absorption spectral changes of LYSO–colloidal TiO 2 nanoparticles using Benesi–Hildebrand equation. The number of binding sites and the apparent binding constant were calculated from relevant fluorescence data. Based on Forster's non-radiation energy transfer theory, distance between the donor (LYSO) and the acceptor (TiO 2) has also been calculated. The conformational changes of LYSO have been analyzed by means of FT-IR and synchronous fluorescence spectroscopy. In addition, the effect of metal ions on the binding constants of LYSO–TiO 2 complex has also been discussed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
