Abstract
A hydrogen production system, coupling D-maltose hydrolysis by glucoamylase and glucose dehydrogenase (GDH) and hydrogen production with Pt colloid catalyst using light-induced photosensitization of Mg chlorophyll a (Mg Chl-a), was developed. Hydrogen gas was continuously produced when the reaction mixture containing D-maltose, glucoamylase, GDH, nicotinamide adenine dinucreotide (NAD+), Mg Chl-a, methylviologen (MV2+) and Pt colloid was irradiated by visible light. The amount of hydrogen production was estimated to be 5.0 μmol after 4 h irradiation and the yield of conversion of D-maltose to hydrogen gas was about 1.8%. The quantum yield was 3.1%.
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