Abstract
Abstract 6-Formylpterin is one of the oxidized pterins that accumulate in the white skin patches of patients affected by vitiligo. Studies of photoinduced generation of hydrogen peroxide (H2O2 ) and superoxide anion radical (O2• ) by formylpterin in acidic and alkaline aqueous solutions at 350 nm and room temperature have been performed. The photochemical processes were followed by UV-V1S spectrophotometry and enzymatic methods for H2O2 and O2•- determination. When formylpterin is irradiated a red intermediate, that has been proposed to be 6-carboxy- 5,8-dihydropterin, is generated in an O2 -independent process. In the presence of air that intermediate reacts with O2 to yield H2O2 and 6-carboxyptcrin. In anaerobic conditions it undergoes a thermal O2-independent reaction which leads to a non-identified product without generation of H202 . The rate constants of such a process were determined to be 0.011 min 1 and 0.044 min1 at pH 5.4 and 10.5, respectively. (O2 · ), is also generated during photooxidation of formylpterin, probably as a consequence of the electron transfer from the red intermediate to O2 . The biomedical importance of photoinduced generation of H2O2 and O2• is discussed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have