Abstract
Photoinduced cleavage reactions by the rhodium complex tris(4,7-diphenyl-1,10-phenanthroline)rhodium(III) {Rh(DIP) 3 3+} with three RNA hairpins, r(GGGGU UCGCUC CACCA) (16 nucleotide, tetraloop Ala2), r(GGGGCUAUAGCUCUAGCUC CACCA) (24 nucleotide, microhelix Ala), and r(GGCGGUUAGAUAUCGCC) (17 nucleotide, 790 loop), and full-length (1542 nucleotide) 16S rRNA from Escherichia coli were investigated. The cleavage reactions were monitored by gel electrophoresis and the sites of cleavage by Rh(DIP) 3 3+ were determined by comparisons with chemical or enzymatic sequencing reactions. In general, RNA backbone scission by the metal complex was induced at G ·U mismatches and at exposed G residues. The cleavage activity was observed on the three small RNA hairpins as well as on the isolated 1542-nucleotide ribosomal RNA.
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