Abstract
The application of intact-cell mass spectrometry (ICM) by MALDI-TOF mass spectrometry to achieve direct protein-profiling of bacterial species is now well established. However this methodology has not to our knowledge been applied to the analysis of mammalian cells in routine culture. Here, we describe a novel application of ICM by which we have identified proteins in intact cells from two lines representative of pancreatic islet α and β cells. Adherent αtc1 clone 9 and βTC6 F7 cells were harvested into PBS using enzyme-free dissociation buffer before 1 μL of cell suspension was spotted onto MALDI plates. Cells were overlaid with sinapinic acid then washed with pure water before application of a final coat of sinapinic acid. Data in the 2,000-20,000 m/z range were acquired in linear mode on a Voyager DE-Pro mass spectrometer [1].
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