Abstract

A description is given of apparatus suitable for photographically observing the changes of mammalian erythrocytes in ultrasonic fields at frequencies from 250 to 1000 kc. These studies were undertaken to investigate the possibility of mechanical resonant modes of vibrations of the erythrocytes. The apparatus consists of an electronic generator, two barium titanite transducers, a microscope, a specimen exposure slide and holder, a camera, and photoflash unit. Photographs were taken of human, sheep, bovine, and rat erythrocytes showing the effects of frequency changes as well as changes in intensity level. Sensitivities were obtained by determining the percentage of red blood cells affected for various intensities at a given frequency. At this frequency, all the sensitivities for the four species were plotted as a function of intensity. Relative sensitivities were obtained by taking the ratios of the slopes of the best straight lines for three species with respect to the slope for the fourth species. These ratios were then plotted as a function of frequency. Results indicate the existence of several mechanical resonant modes in the frequency range investigated.

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