Abstract
As an emerging analytical technology, photoelectrochemical (PEC) biosensor have attracted widespread attentions for detection of various targets. Herein, a simple, sensitive and selective PEC immunosensor was constructed for RNA methylation detection, where g-C3N4/Bi2S3 with improved photoelectric properties was used as the photoactive material, anti-m6A antibody was employed as target recognition molecule, and the target of m6A RNA was also adopted as photocurrent inhibitor. Under optimal experimental conditions, the immunosensor presented wide linear range from 0.1 − 50 nM and low detection limit of 0.025 nM (S/N = 3). The immunosensor also possessed high stability. Considering the growing threat of heavy metal and antibiotic pollution to food security, the applicability of the develop method was assessed by investigating the effect of Pb2+-antibiotics binary pollutants on RNA methylation level in the leaves and roots of rice seedlings.
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