Photoelectrochemical immunosensor for chloramphenicol detection based on cation exchange reaction-mediacted photocurrent enhancement of ZnIn2S4/TiO2/Ti3C2 MXene coupled with controlled-release strategy.

Abstract

Aphotocurrent enhancing photoelectrochemical (PEC) immunosensor was developedfor chloramphenicol (CAP) detection based on cation exchange reaction. The efficient split-type PEC immunosensor combined with controlled-release strategy was established using the ZnIn2S4/TiO2/Ti3C2 MXene (ZIS/T/M) composite as the photoactive material and CuO as the signal response probe. In the presence of target CAP, CuO-labeled CAP antibody (CuO-mAb) was introduced onto the microplate via a competitive-type immunoassay. Under acidic conditions, a large amount of Cu2+ released from CuO-mAb, which triggered a cation exchange reaction with the Zn2+ in ZIS/T/M-modified photoelectrode to generate CuxS, resulting in enhancingthe photocurrent. As a result, the quantitative detection of CAP was achieved by detecting the photocurrent change. Under optimized conditions, the linear range of the sensor was 1pg/mL to 50ng/mL, and the detection limit was 0.24pg/mL. The excellent PEC behavior of ZIS/T/M composite could be attributed to the fact that heterojunction formation improved the migration and separation of thephotocarrier. Additionally, by virtue of the photocurrent-enhancing strategy via cation exchange reaction and the controlled releasing signal amplification method of ion, the PEC immunosensor has high sensitivity and satisfactory accuracy, offering great potential applications in the determinationof CAP.