Abstract
Background: Photodynamic therapy (PDT) is the generation of cytotoxic agents through the dynamic interaction between a photosensitizer excited by light at a specific wavelength. When associated with phthalocyanines, they are efficient in incorporating target cells and exhibiting high rates of triplet generation. This study aimed to characterize PDT associated with the phototherapeutic agent Phthalomethyl D, developed by the authors, in the process of repair, healing and immune improvement for possible application against SARS-CoV-2. Methods: Sixty-nine mice were used, divided into 2 groups: GI, treated with ILIB laser, without a phototherapeutic agent, and subjected to surgery for viral induction; GII, same as GI plus association of Phthalomethyl D. They were divided into subgroups and reevaluated at 7, 14, and 21 days, and then divided into 3 subgroups of 6 animals each, subjected to treatment at 24 h, 48 h, 72 h, 5 and 7 days. Results: Both groups had a high rate of partial incision closure and acute inflammatory control. Microscopically, there was a greater amount of amorphous fundamental substance, fibrocytes, fibroblasts, and giant cells and reduction in the number of keratinocytes, in the amount of keratin, and epidermal thickness in GII than in GI. Conclusions: PDT with Phthalomethyl D stimulates the processes of healing/repair and immunomodulation during viral infection, initially favoring the inflammatory response and, after 21 days, contributing to the anti-inflammatory response profile, making this approach possible in the treatment of individuals infected with SARS-CoV-2.
Highlights
The recent global new coronavirus (SARS-CoV-2) pandemic has frightened communities around the world due to its high rates of transmissibility and death, leading to countless economic, psychosocial, and catastrophic consequences [1]
This study aimed to use experimental models of poxvirus infection to determine whether the use of photodynamic therapy (PDT) associated with the phototherapeutic agent Phthalomethyl D, developed by the authors, would be effective in the process of repair, healing and immune improvement induced by poxviruses in mice
At 48 h after surgery, gross examination of incision closure revealed the presence of blood clots in 60% of mice in group II (PDT + Phthalomethyl D)
Summary
The recent global new coronavirus (SARS-CoV-2) pandemic has frightened communities around the world due to its high rates of transmissibility and death, leading to countless economic, psychosocial, and catastrophic consequences [1] Because it is an unknown, recent and devastating pathology, there is no effective treatment or prevention for SARS-CoV-2 infection, requiring rapid mobilization and investigation of potential substances and clinical tools, including photodynamic therapy (PDT) [2] [3] [4]. Methods: Sixty-nine mice were used, divided into 2 groups: GI, treated with ILIB laser, without a phototherapeutic agent, and subjected to surgery for viral induction; GII, same as GI plus association of Phthalomethyl D They were divided into subgroups and reevaluated at 7, 14, and 21 days, and divided into 3 subgroups of 6 animals each, subjected to treatment at 24 h, 48 h, 72 h, 5 and 7 days. Conclusions: PDT with Phthalomethyl D stimulates the processes of healing/repair and immunomodulation during viral infection, initially favoring the inflammatory response and, after 21 days, contributing to the an-
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