Abstract
Efficacy of photodynamic therapy with intraperitoneal introduction photosensitizer Amidoaminchlorine on rat sarcoma M1 model was studied. The aim of this study was to determine minimum effective dose of photosensitizer and parameters of laser radiation for achievement of tumor full regress. Dynamics of photosensitizer accumulation in tumor and healthy tissues was studied to definite the time of photodynamic therapy. Antitumor efficacy different doses of photosensitizer and various parameters of laser radiation were investigated. As a result, optimal term of photodynamic therapy, photosensitizer minimum effective dose and parameters of laser irradiation were established.
Highlights
Dynamics of photosensitizer accumulation in tumor and healthy tissues was studied to definite the time of photodynamic therapy
Ʉɪɨɦɟ ɩɪɹɦɨɝɨ ɰɢɬɨɬɨɤɫɢɱɟɫɤɨɝɨ ɜɨɡɞɟɣɫɬɜɢɹ ɎȾɌ ɧɚ ɨɩɭɯɨɥɟɜɵɟ ɤɥɟɬɤɢ ɜɚɠɧɭɸ ɪɨɥɶ ɜ ɞɟɫɬɪɭɤɰɢɢ ɧɨɜɨɨɛɪɚɡɨɜɚɧɢɹ ɢɝɪɚɸɬ: ɧɚɪɭɲɟɧɢɟ ɤɪɨɜɨɫɧɚɛɠɟɧɢɹ ɡɚ ɫɱɟɬ ɩɨɜɪɟɠɞɟɧɢɹ ɷɧɞɨɬɟɥɢɹ ɤɪɨɜɟɧɨɫɧɵɯ ɫɨɫɭɞɨɜ ɨɩɭɯɨɥɟɜɨɣ ɬɤɚɧɢ; ɰɢɬɨɤɢɧɨɜɵɟ ɪɟɚɤɰɢɢ, ɨɛɭɫɥɨɜɥɟɧɧɵɟ ɫɬɢɦɭɥɹɰɢɟɣ ɩɪɨɞɭɤɰɢɢ ɮɚɤɬɨɪɚ ɧɟɤɪɨɡɚ ɨɩɭɯɨɥɢ, ɚɤɬɢɜɚɰɢɟɣ ɦɚɤɪɨɮɚɝɨɜ, ɥɟɣɤɨɰɢɬɨɜ ɢ ɥɢɦɮɨɰɢɬɨɜ. ɉɪɨɝɪɟɫɫ ɎȾɌ ɨɩɭɯɨɥɟɣ ɫɜɹɡɚɧ ɫ ɫɨɡɞɚɧɢɟɦ ɷɮɮɟɤɬɢɜɧɵɯ Ɏɋ, ɨɛɟɫɩɟɱɢɜɚɸɳɢɯ ɞɨɫɬɚɬɨɱɧɵɣ ɬɟɪɚɩɟɜɬɢɱɟɫɤɢɣ ɷɮɮɟɤɬ ɩɪɢ ɦɢɧɢɦɚɥɶɧɨɦ ɩɨɜɪɟɠɞɟɧɢɢ ɨɤɪɭɠɚɸɳɢɯ ɡɞɨɪɨɜɵɯ ɬɤɚɧɟɣ ɢ ɨɬɫɭɬɫɬɜɢɢ ɨɛɳɟɬɨɤɫɢɱɟɫɤɨɝɨ ɞɟɣɫɬɜɢɹ. ȼ ɧɚɫɬɨɹɳɟɟ ɜɪɟɦɹ ɛɨɥɶɲɨɣ ɢɧɬɟɪɟɫ ɜ ɎȾɌ ɡɥɨɤɚɱɟɫɬɜɟɧɧɵɯ ɧɨɜɨɨɛɪɚɡɨɜɚɧɢɣ ɜ ɤɚɱɟɫɬɜɟ Ɏɋ ɜɵɡɵɜɚɸɬ ɩɪɨɢɡɜɨɞɧɵɟ ɪɹɞɚ ɯɥɨɪɨɮɢɥɥɚ [15]. ȼɧɨɜɶ ɫɨɡɞɚɧɧɵɣ ɚɦɢɞɨɚɦɢɧɯɥɨɪɢɧ ɹɜɥɹɟɬɫɹ ɯɢɦɢɱɟɫɤɨɣ ɦɨɞɢɮɢɤɚɰɢɟɣ ɩɟɪɢɮɟɪɢɱɟɫɤɢɯ ɡɚɦɟɫɬɢɬɟɥɟɣ ɯɥɨɪɢɧɚ ɟ6. ɋɩɟɤɬɪɚɥɶɧɵɟ ɯɚɪɚɤɬɟɪɢɫɬɢɤɢ ɚɦɢɞɨɚɦɢɧɯɥɨɪɢɧɚ ɩɨɡɜɨɥɹɸɬ ɢɫɩɨɥɶɡɨɜɚɬɶ ɟɝɨ ɫ ɬɟɦɢ ɠɟ ɥɚɡɟɪɚɦɢ, ɤɨɬɨɪɵɟ ɢɫɩɨɥɶɡɭɸɬɫɹ ɞɥɹ ɞɪɭɝɢɯ Ɏɋ ɯɥɨɪɢɧɨɜɨɝɨ ɪɹɞɚ, ɬ. ɟ. ɫ ɦɚɤɫɢɦɭɦɨɦ ɢɡɥɭɱɟɧɢɹ ɩɪɢ ɞɥɢɧɟ ɜɨɥɧɵ 662 ɧɦ [1; 5; 16; 17]
Ⱦɨ 7 ɫɭɬɨɤ ɢɫɫɥɟɞɨɜɚɧɢɹ ɨɬɦɟɱɚɥɚɫɶ ɩɨɥɧɚɹ ɪɟɝɪɟɫɫɢɹ ɨɩɭɯɨɥɢ ɭ ɜɫɟɯ ɠɢɜɨɬɧɵɯ ɜ ɝɪɭɩɩɟ. ɇɚ 21 ɫɭɬɤɢ ɢɫɫɥɟɞɨɜɚɧɢɹ ɌɊɈ ɫɨɫɬɚɜɥɹɥɨ 97,9 % ɩɪɢ 50 % ɉɊ ɨɩɭɯɨɥɢ, ɤɨɷɮɮɢɰɢɟɧɬ ɚɛɫɨɥɸɬɧɨɝɨ ɩɪɢɪɨɫɬɚ ɭ ɠɢɜɨɬɧɵɯ ɫ ɪɨɫɬɨɦ ɨɩɭɯɨɥɢ (3,79±2,68) ɨɬɥɢɱɚɥɫɹ ɨɬ ɤɨɷɮɮɢɰɢɟɧɬɚ ɜ ɝɪɭɩɩɟ 3 ɢ ɡɧɚɱɢɬɟɥɶɧɨ ɨɬɥɢɱɚɥɫɹ ɨɬ ɤɨɧɬɪɨɥɹ (p
Summary
Dynamics of photosensitizer accumulation in tumor and healthy tissues was studied to definite the time of photodynamic therapy. Ɂɡɭɱɟɧɢɟ ɞɢɧɚɦɢɤɢ ɧɚɤɨɩɥɟɧɢɹ Ɏɋ ɜ ɨɩɭɯɨɥɢ ɢ ɡɞɨɪɨɜɨɣ ɬɤɚɧɢ ɤɪɵɫ Ⱦɥɹ ɢɡɭɱɟɧɢɹ ɞɢɧɚɦɢɤɢ ɧɚɤɨɩɥɟɧɢɹ ɚɦɢɞɨɚɦɢɧɯɥɨɪɢɧ ɜɜɨɞɢɥɢ ɤɪɵɫɚɦ ɫ ɫɚɪɤɨɦɨɣ Ɇ-1 ɜɧɭɬɪɢɛɪɸɲɢɧɧɨ ɜ ɞɨɡɟ 2,5 ɦɝ/ɤɝ. Ɂɡ ɞɚɧɧɵɯ, ɩɪɟɞɫɬɚɜɥɟɧɧɵɯ ɧɚ ɪɢɫɭɧɤɟ, ɜɢɞɧɨ, ɱɬɨ ɜ ɬɟɱɟɧɢɟ 5,5 ɱ ɩɨɫɥɟ ɜɧɭɬɪɢɛɪɸɲɢɧɧɨɝɨ ɜɜɟɞɟɧɢɹ ɚɦɢɞɨɚɦɢɧɯɥɨɪɢɧɚ ɜ ɞɨɡɟ 2,5 ɦɝ/ɤɝ ɩɪɨɢɫɯɨɞɢɬ ɩɨɫɬɟɩɟɧɧɨɟ ɭɜɟɥɢɱɟɧɢɟ ɭɪɨɜɧɹ ɧɚɤɨɩɥɟɧɢɹ ɟɝɨ ɤɚɤ ɜ ɨɩɭɯɨɥɢ, ɬɚɤ ɢ ɜ ɡɞɨɪɨɜɨɣ ɬɤɚɧɢ.
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