Photodynamic therapy inhibits cell growth of human cholangiocarcinoma QBC939 cells in vitro

Abstract

Objective To investigate the effects of hematoporphyrin derivative-mediated photodynamic therapy (HPD-PDT) on cell growth in human cholangiocarcinoma in vitro and in vivo, as well as the underlying mechanisms of these effects. Methods 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate growth status of human cholangiocarcinoma cell line (QBC939). Hoechst 33258 staining and flow cytometry assays were applied to determine cell apoptosis. Western blotting analysis was performed to detect the release of cytochrome c in QBC939 cells, and caspases enzymatic assay was used to investigate the activation of caspase-3, -8, and -9. Results HPD-PDT inhibits QBC939 cell growth via cell apoptosis in vitro, and initiates cell mitochondria apoptosis pathway by the release of cytochrome c and the activation of caspase-9 and -3. Conclusion HPD-PDT inhibits tumor growth and induces cell apoptosis of human cholangiocarcinoma, suggesting that HPD-PDT is useful in cholangiocarcinoma therapy. Key words: Hematoporphyrin derivative; Photodynamic therapy; Apoptosis; Cholangiocarcinoma