Abstract
A microsensor system to investigate photodynamic therapy of cancer cells in vitro was established. Monitoring of the cellular respiration during and after photodynamic therapy was demonstrated. The system comprises a sensor chip with cell culture chamber, allowing photodynamic treatment by laser light and simultaneous monitoring of metabolic parameter using electrochemical sensors in situ. The sensor chip is integrated into a fluorescence microscope allowing the detection of fluorescence emitted by the photosensitizer as well as to detect fluorescence from additional marker providing optical measurements in parallel to the microsensor readings.
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