Photodynamic effectiveness of laser diode combined with ozone to reduce STaphylicoccus aureus biofilm with exogenous chlorophyll of Dracaena angustifolia leaves

S D Astuti,N D Drantantiyas,A P Putra,S Suhariningsih,A Syahrom,P S Puspita

doi:10.24931/2413-9432-2019-8-2-4-13

Abstract

Photodynamic inactivation is an effective treatment that uses light irradiation, photosensitizer and oxygen. The aim of this study was to determine photodynamic effectiveness of laser diode combined with ozone to reduce Staphylococcus aureus biofilm using exogenous chlorophyll (Chlo). The chlorophyll was extracted from leave of Dracaena angustifolia. To determine the antibacterial effect of S. aureus biofilm treatments, samples were separated into Chlo, Laser, Chlo+Laser, Ozone, Ozone+Laser, Chlo+Ozone+Laser categories. The data were analyzed using ANOVA test. The result of this study showed that Chlo+Ozone+Laser combine treatment at 20 s exposure of ozone with 4 min of irradiation time lead to 80.26 % reduction of biofilm activity, which was the highest efficacy of all the treatment groups. The combination of laser, chlorophyll and lower ozone concentration increases the effectiveness of photodynamic inactivation.

Highlights

Biofilms are colonies of bacteria that produce a protective matrix layer called extracellular polymeric substance (EPS) and have higher virulence, resistance, and pathogenic properties [1,2]
There is a possibility of biofilm having the ability to reduce production of hydrogen peroxide (H2O2) which is a precursor of toxic molecules when DNA-protein synthesis changes [5]
This study aimed to determine photodynamic effectiveness of laser diode combined with ozone to reduce Staphylococcus aureus biofilm using exogenous chlorophyll

Summary

Introduction

Biofilms are colonies of bacteria that produce a protective matrix layer called extracellular polymeric substance (EPS) and have higher virulence, resistance, and pathogenic properties [1,2]. The PDI mechanism starts from the absorption of light the wavelength of which corresponds to the absorbance of photosensitizer. It can produce reactive oxygen species (ROS) through type I and II photochemical processes [7]. A previous report related of PDI using silver nanoparticles as photosensitizer and laser diode with an output of 450.00 ± 22.34 nm and 53.16 ± 0.01 mW. This combination could decrease the surviving biofilm compared to the laser diode itself by 64.48 ± 0.07% against 7.07 ± 0.23% at 6.13 ± 0.002 J/cm, respectively [8]

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