Abstract
Photocytotoxic activity sensitized by 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]chlorin (TAPC) was investigated in Candida albicans under different culture conditions. Planktonic cells incubated with 2.5 μM TAPC were eradicated after 5 min irradiation with white light. Studies in the presence of reactive oxygen species scavengers indicated the involvement of mainly a type II mechanism. Furthermore, cell growth of C. albicans was suppressed in the presence of 5 μM TAPC. A decrease in pseudohyphae survival of 5 log was found after 30 min irradiation. However, the photokilling of this virulence factor reached a 1.5 log reduction in human serum. The uptake of TAPC by pseudohyphae decreased in serum due to the interaction of TAPC with albumin. The binding constant of the TAPC-albumin complex was ~104 M−1, while the bimolecular quenching rate constant was ~1012 s−1 M−1, indicating that this process occurred through a static process. Thus, the photoinactivation of C. albicans was considerably decreased in the presence of albumin. A reduction of 2 log in cell survival was observed using 4.5% albumin and 30 min irradiation. The results allow optimizing the best conditions to inactivate C. albicans under different culture conditions.
Highlights
In the past three decades, significant growth of invasive and mucocutaneous fungal infections was observed with a high risk to human health
Eradication of C. albicans planktonic cells was possible at low TAPC concentrations and short irradiation times
This chlorin was able to suspend the growth of yeasts in the culture medium by means of continuous light irradiation
Summary
In the past three decades, significant growth of invasive and mucocutaneous fungal infections was observed with a high risk to human health. This situation is mainly caused by the increase in resistance to drugs by fungi and a deficiency of new strategies to control mycosis [1,2]. This problem is increasing due to the inappropriate use of antimicrobials. Candida albicans is the most prevalent species isolated from humans regardless of the health condition of the patient
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