Abstract
In the presence of ordinary visible light from an electric bulb, methylene blue was found to exert a rapid bactericidal action on certain bacteria which survived the same dye even in higher concentration in the absence of lamplight.1 At the same time, gram negative bacilli were found to be highly resistant to this action of methylene blue. In continuation of a systematic study of photo-dynamic action of dyes on bacteria, various other common dyes have been chosen, and tests with representative gram positive and gram negative organisms repeated. The result of such a study is hereby presented.Saline solutions of eosin, mercurochrome, acid fuchsin, basic fuchsin, and fluorescein, and a commercial 2% solution of trypa-flavine were used. Bacteria were grown on either blood- or plain meat-infusion agar for 24 hours and were then suspended in saline. Except in the case of trypaflavine, which was diluted with the suspension to the desired concentrations, suspensions were added to equal parts of dyes in the differ...
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