Abstract
A simple, environmentally benign and low-cost method for the synthesis of different-shaped gold nanoparticles using silk fibroin (SF) under ultraviolet (UV-B) radiation has been reported in this work. No additives such as organic solvents, surfactants, external reducing, stabilizing agents or high temperature were used for the synthesis of the particles. SF acts as a reducing and subsequently, stabilizing agent. The UV-Visible (UV-Vis) absorption spectra support the formation of different-shaped gold nanoparticles by displaying transverse and longitudinal surface plasmon bands at different wavelengths. The size and shape evolution of the nanoparticles was studied using the transmission electron microscope (TEM) to confirm the formation of different-shaped particles. Nano-crystalline structure and FCC crystal family of gold were confirmed by X-ray diffraction (XRD) measurement study. Furthermore, the proliferative activities of the synthesized colloidal gold nanoparticles were tested against lymphocyte and Jurkat cancer cell lines. The obtained results proved that the biogenic different-shaped AuNPs showed better activity in comparison with the spherical-shaped gold nanoparticles.
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