Abstract
Carba-NAD+and pseudocarba-NAD+, and their 8-azidoadenosyl derivatives, were found to be good competitive inhibitors of calf spleen NAD+glycohydrolase. The 8-azido compounds, tested as photoaffinity labels, inhibited the enzyme in a light- and time-dependent manner; this inhibition could be prevented by 3-aminopyridine adenine dinucleotide (n3PdAD+), a competitive inhibitor of NAD+glycohydrolase. Irradiation in the presence of the [3H]-labeled 8-azido-carba-NAD+derivative resulted in an irreversible incorporation of the radioactivity into the enzyme that could be largely prevented by addition of n3PdAD+. These results indicate that carbocyclic-analogs of NAD+will be useful in identifying the substrate binding site of NAD+glycohydrolase.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
