Abstract
BackgroundAmong various extra stimuli of humans, ultraviolet (UV) has been the most studied factor because it arouses not only internal but also external irritation in the body. UVA, one type of UV rays, has a wavelength between 320 and 400 nm and capacity to penetrate the skin dermal layer. Therefore, studies on how to reduce UVA-induced maleficence have been investigated vibrantly. Angelic acid has been demonstrated to aid in wound healing and exhibited sedative and psychotropic properties. But there have not been sufficient reports whether angelic acid has potential properties in the cosmeceutical aspect.MethodsTo investigate protective effects of angelic acid on UVA-induced oxidative stress and disruption of extracellular matrix, researchers analyzed cell proliferation rate, intracellular reactive oxygen species (ROS) scavenging capacity, cellular senescence, transcriptional activity of activating protein-1 (AP-1) transcription factor, and gene expression of antioxidant enzymes and connective tissue-related proteins.ResultsPretreatment of angelic acid in normal human dermal fibroblasts (NHDFs) showed protective effect on UVA-induced proliferative inhibition. Via estimating ROS scavenging activity, angelic acid represented a scavenging effect of excessive increased intracellular ROS which is induced by UVA irradiation. Through quantitative real-time polymerase reaction, antioxidant enzyme and extracellular matrix (ECM)-related protein coded gene expressions were analyzed. Analysis of senescent cell and AP-1 promoter activity by beta-galactosidase assay and luciferase reporter gene assay, respectively, indicated how angelic acid regulates cellular mechanisms associated with connective tissue density.ConclusionsThrough the present study, researchers verify that angelic acid has dermal protective effect against UVA and suggest angelic acid as an efficacious cosmetic material preventing dermal cellular damages.
Highlights
Among various extra stimuli of humans, ultraviolet (UV) has been the most studied factor because it arouses internal and external irritation in the body
The senescence-associated β-galactosidase (SA-β-gal) staining kit was obtained from Biovision (Milpitas, CA, USA), and the manufactured primers for the quantitative real-time polymerase chain reaction (RT-qPCR) analysis were purchased from Macrogen (Seoul, Korea)
Pretreatment with angelic acid inhibits UVA-induced cytotoxicity in HDFs Prior to estimating protective effect on UVA-induced cytotoxicity, researchers examine whether angelic acid has cytotoxicity as the concentration range is tested
Summary
Among various extra stimuli of humans, ultraviolet (UV) has been the most studied factor because it arouses internal and external irritation in the body. Angelic acid is a substance found in the essential oil of Anthemis nobilis, a plant from the Asteraceae family, or Angelica from the Apiaceae family, and it exists in an ester form (Ernest, 2006; Craker & Simon, 1986). It is isolated from carrots, Euphorbia species and Alkanna tinctoria (Sonobe et al, 1981; Sosath et al, 1988; Papageorgiou, 1978). The present study is designed to determine the role of angelic acid on UVA-induced oxidative stress and dermal senescence in normal human dermal fibroblasts (NHDFs) by assessing its excessive ROS scavenging capacity and ECM deterioration
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
