Abstract
Photoresponsive polymeric adsorbents containing an azobenzene moiety were prepared, and adsorption behavior of lysozyme onto the adsorbents was studied. In the dark the amount of lysozyme adsorbed increased with increasing hydrophobicity of the surface of the adsorbents. Therefore, it is suggested that hydrophobic interaction between lysozyme and the adsorbents plays an important role in their adsorption behavior. On irradiation with UV light, the amount of lysozyme adsorbed decreased. This result is due to increased polarity of the surface of the adsorbent induced by UV irradiation and the resultant reduction in hydrophobic interaction with lysozyme. When a column chromatography of lysozyme was carried out using the photoresponsive polymeric adsorbent as a packing material, lysozyme was eluted by photoirradiation in a system using water as the single solvent.
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