Abstract
The photoreaction of 9- cis-7,8-dihydrorhodopsin was examined at liquid nitrogen temperatures (−180°C) in order to elucidate the photochemical events in visual pigments. This rhodopsin analog was prepared by incubating 9- cis-7,8-dihydroretinal with bovine opsin in the dark. 9- cis-7,8-Dihydrorhodopsin ( λ max = 427 nm) was cooled to −180°C, and then irradiated at −180°C with a 390 nm light, resulting in formation of its bathochromic product ( λ max = roughly 465 nm). This result indicates that the presence of four double-bonds adjacent to the Schiff base nitrogen is sufficient to allow formation of a bathochromic product. Thus, the mechanism of formation of bathorhodopsin (in bovine rhodopsin system) may be considered as some change of the interaction between the conjugated double-bond system from C-9 to the Schiff base nitrogen and its surrounding charges in opsin, caused by rotation of 11–12 double-bond.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Bioenergetics
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