Photochemical Behavior of Lysozyme-4-(2-pyridylazo)-resorcinol Polymolecular Complex and Its Analytical Applications

Abstract

It was first found that 4-(2-pyridylazo)-resorcinol (PAR) can combine with lysozyme forming a polymolecular complex, with the molar ratio of 1:12 (lysozyme/PAR) in the medium of acetate buffer (pH 6.0). The fluorescence spectra suggested that PAR molecules may produce self-aggregation, and it was suggested that there are interactions between lysozyme and PAR possibly mainly on the cooperation of hydrophobic, electrostatic actions and chelate bond. The coordination complex of lysozyme-PAR can cause an enhanced resonance light scattering (RLS) signal with the scattering peak located at 521 nm. The increment of RLS intensity was linear with the concentration of lysozyme over the range from 1 ng mL(-1) to 30.0 mug mL(-1), with the limit of detection of 0.3 ng mL(-1) (3sigma, n = 5). Effect of experimental conditions, including temperature, pH, concentration of PAR, and the ionic strength on RLS were also tested. The proposed RLS method was successfully applied to analyze lysozyme in hen egg white, human saliva and tear samples without any special pretreatment. Compared with other methods, the proposed procedure is of high sensitivity and selectivity.