Photocatalytic antibacterial activity of TiO2–SiO2 thin films: The effect of composition on cell adhesion and antibacterial activity

Abstract

• The effect of composition of TiO 2 –SiO 2 thin films on cell adhesion was studied. • The effect of surface composition on surface free energy was studied. • SiO 2 addition favors for cell adhesion. • Strong relation was found between the bacterial adhesion and antibacterial activity. Colloidal solutions of SiO 2 –TiO 2 mixed oxides having different SiO 2 contents were synthesized by sol–gel technique and thin films were deposited over glass slides by using dip coating technique. The samples were characterized by XRD, SEM, AFM and methylene blue adsorption methods. The surface free energy of thin films was measured by sessile drop method by using diiodomethane, glycerol and water as probe liquids. The photocatalytic antibacterial activity of the samples was determined for Escherichia coli XL1-blue by using artificial solar irradiation. The surface characterization studies revealed the presence of small crystallites of anatase phase, enhanced dispersion of TiO 2 particles and increasing surface free energy at elevated SiO 2 content. Highest photocatalytic antibacterial activity was obtained over 92 wt% SiO 2 containing sample and strong correlation was found between the bacterial adhesion and antibacterial activity. Surface-adhered E. coli cells having direct contact with TiO 2 particles were inactivated at a higher rate than suspended cells.