Abstract
Functional chemical micropatterns were fabricated by exploiting the photobleachingof dye-coupled species near methacrylate self-assembled monolayers. Using thisapproach we have demonstrated that multiple chemistries can be coupledto the monolayer using a standard fluorescence microscope. The surface boundfunctional groups remain active and patterns with feature sizes down to3 µm can be readily achieved with excellent signal-to-noise ratio. Control over the ligand bindingdensity was demonstrated to illustrate the convenient route provided by this platform forfabricating complex spatial gradients in ligand density.
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