Photobiomodulation in human neuroblastoma under oxidative stress

Abstract

AbstractBackgroundWith the increase in life expectancy, the number of people with neurodegenerative diseases such as Alzheimer’s Disease (AD) is rising rapidly. Considering the pathways of AD, one of its fundamental processes is oxidative stress, affecting the central nervous system and cell regeneration. Interestingly, in the last decades, photobiomodulation has shown positive effects on neural cells that are compromised by AD. Studies have demonstrated that LED (light emitting diode) irradiation regulates cell function, stimulating cell proliferation, promoting biomodulation in the cytoskeleton, and reducing apoptosis. To contribute with the elucidation of the importance of photobiomodulation in AD, this study aims to evaluate its neuroprotective effect, comparing different energy density of LED irradiating in SH‐SY5Y cells (human neuroblastoma) under the effect of oxidative stress induced by hydrogen peroxide (H2O2).MethodSH‐SY5Y cells (human neuroblastoma) were cultivated in DMEM/F12 medium (Dulbecco’s modified Eagles medium ‐ Gibco), supplemented with 10% fetal bovine serum and 1% antibiotic and antimycotic, at 37°C, in an atmosphere of 5% CO2. Cells were plated in 24‐well plates. After cell adhesion, cells were irradiated with LED at 660 nm (LED Biopdi/Irrad‐Led5), at energy densities of 1 J/cm2, 3 J/cm2, 5 J/cm2 and 10 J/ cm2. 24 h after irradiation, oxidative stress was induced with 200 μM H2O2 for 24h (this parameter had been previously determined by ROSSATO, R. C. et al, 2021).ResultOnce cells are irradiated and stressed, a considerable statistic difference is observed for all energy densities evaluated, compared to the stressed‐only group (H2O2). Importantly, the energy density of 3 J/cm2 was the lowest one that obtained better statistical increase in mithocondrial activity (p < 0.0001).ConclusionIt was possible to determine and compare the energy densities of LED irradiation to promote cellular photobiomodulation, inducing mithocondrial activity on neubroblastoma. Importantly, this study demonstrated the neuroprotective effect of LED in an in vitro model of AD oxidative stress, approaching novel concepts and a preventive tool.ROSSATO, R. C. et al. Neuroprotective effects of taurine on SH‐SY5Y cells under hydrocortisone induced stress. Research, Society and Development, v. 10, n. 9, p. e55510918426‐e55510918426, 2021