Abstract
Human adipose-derived stem cells (hASCs) have the potential to differentiate into several different cell types including osteoblasts. Photobiomodulation (PBM) or low level laser therapy (LLLT) using red or near-infrared wavelengths has been reported to have effects on both proliferation and osteogenic differentiation of stem cells. We examined the effects of delivering four different wavelengths (420 nm, 540 nm, 660 nm, 810 nm) at the same dose (3 J/cm2) five times (every two days) on hASCs cultured in osteogenic medium over three weeks. We measured expression of the following transcription factors by RT-PCR: RUNX2, osterix, and the osteoblast protein, osteocalcin. The 420 nm and 540 nm wavelengths were more effective in stimulating osteoblast differentiation compared to 660 nm and 810 nm. Intracellular calcium was higher after 420 nm and 540 nm, and could be inhibited by capsazepine and SKF96365, which also inhibited osteogenic differentiation. We hypothesize that activation of light-gated calcium ion channels by blue and green light could explain our results.
Highlights
Wang, Yuguang, Ying-Ying Huang, Yong Wang, Peijun Lyu, and Michael R
This tissue represents an abundant and accessible source of adult stem cells that can be purified from the lipoaspirate, with the ability to differentiate along multiple lineage pathways2. Human adipose-derived stem cells (hASCs) have been shown to be very similar to bone marrow-derived mesenchymal stem cells (BMDMSC)[3]
We found that the RUNX2 level of the green light group at all three time points were higher than red, near infrared and osteogenic medium (OM) groups
Summary
Yuguang, Ying-Ying Huang, Yong Wang, Peijun Lyu, and Michael R. “Photobiomodulation (blue and Green Light) Encourages Osteoblastic-Differentiation of Human Adipose-Derived Stem Cells: Role of Intracellular Calcium and Light-Gated Ion Channels.”. Human adipose-derived stem cells (hASCs) have the potential to differentiate into several different cell types including osteoblasts. Adipose tissue is usually isolated in the form of fat removed during liposuction procedures This tissue represents an abundant and accessible source of adult stem cells that can be purified from the lipoaspirate, with the ability to differentiate along multiple lineage pathways. The autologous bone graft procedure has limitations including donor site morbidity, limited amounts, and a requirement for a second surgical procedure To overcome these limitations, researchers have proposed the use of HADSC to provide a source of cells that can differentiate and proliferate into osteogenic cells (osteoblasts) under the influence of the appropriate molecular signals[6]. In addition to the correct scaffold exogenous growth factors www.nature.com/scientificreports/
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