Abstract
Summary The aim of this study was to obtain photoautotrophic suspension cultures from Euphorbia characias L., a Mediterranean species with high isoprenoid content. Calluses were initiated in photomixotrophy on a modified Murashige and Skoog (MS) medium supplemented with NAA and BAP. Selection and subculture led to chlorophyllous and friable calluses which were used to initiate suspension cultures. Batch cultures were performed in 250 ml closed conical flasks and analysed with respect to cell density, sugar consumption and chlorophyll content. Cells in the early stationary phase exhibited good photosynthetic potency, allowing transfer into bioreactors where cells have been grown photoautotrophically since February 1984.
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