Abstract
AbstractA method is described for growing cell suspension cultures of Chenopodium rubrum photoautotrophically for prolonged periods of time. By using a two‐tier culture vessel the growth medium with the cells was separated from the CO2 reservoir. Definite CO2 concentrations were established by a K2CO3/KHCO3 buffer. Photoautotrophic growth in C. rubrum cell suspension cultures was correlated with the CO2 level. At 0.5% CO2 the cell cultures contained 68 μg chlorophyll/g fresh weight and showed an increase in fresh weight of about 80% in 18 days. At 1% CO2 an increase in fresh weight of 165% in 18 days was observed. The chlorophyll content rose up to 84 μg/g fresh weight.The photoautotrophic growth was also greatly influenced by the 2,4‐D content of the medium. Cell growth was enhanced by lowering the auxin concentration. Best growth was attained (210% increase in fresh weight) at 10−8M 2,4‐D.The photosynthetic activity of the cells was measured by the light dependent 14CO2 incorporation. At 0.5% CO2 the cell suspensions assimilated about 100 μmol CO2/mg chlorophyll × h. In the presence of 1% CO2 the light driven assimilation was raised up to 185 μmol CO2/mg chlorophyll × h. In both cases, the dark incorporation of CO2 was merely 1.8% of the values obtained in light.
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