Photoautotrophic cultures of Chlamydomonas reinhardtii: sulfur deficiency, anoxia, and hydrogen production.

Abstract

The aim of this work was a comparative study of S-repleted and S-depleted photoautotrophic cultures of Chlamydomonas reinhardtii under aerobic and anoxic conditions with the main focus on PSII activity. For that we used photobioreactor with short light path connected on-line to PAM fluorometer and cultivated microalgae in twice concentrated HS medium to avoid any uncontrolled limitation by mineral elements. Photoautotrophic cultures grown under Ar + CO2 gas mixture did not reach the same Chl (a + b) concentration as control culture (grown under air + CO2). At pO2 40% of air saturation (96µM O2), the actual quantum yield of PSII started to decrease. Under microaerobic conditions when cultures stopped growing, the most significant changes in PSII function were observed. Maximum quantum yield Fv/Fm decreased significantly along with performance index, PIabs. It was accompanied by increase of fluorescence at J point, Vj. Results indicate that microaerobic conditions are stressful for photoautotrophic cultures. Photoautotrophic cultures of microalgae under S-deprivation in aerobic or anaerobic conditions showed similar behavior as photoheterotrophic ones described earlier. However, photoautotrophic cultures during anaerobiosis establishment did not show sharp "switch off" effect of actual quantum yield. We show also that S-deprivation under air or argon as well as the growth under Ar + CO2 cause significant increase of initial rise of fluorescence, which indicates that PSII and oxygen-evolving complex might be disintegrated.