Photoacoustic spectroscopy allows to make correlations between blood p450 cytochrome and glycemia in type 1 experimental diabetes

Abstract

Diabetes is the eight-cause death worldwide. The cause of death of patients with diabetes is mostly the long-term complications, that are not easy to detect opportunely. In previous studies, we applied photoacoustic spectroscopy (PAS), a non-destructive technique, to detect several components of blood. The goal of the study was to apply the phase-resolved method (PRM), on blood optical absorption spectra obtained by PAS, to analyse blood components in experimental type 1 diabetes. Diabetes was produced in male Wistar rats through the administrations of streptozotocin (STZ). Venous blood samples were obtained one, two, four and eight weeks after STZ. PRM applied to spectra allowed to detect p450 cytochrome. There was a significant and positive correlation between glycaemia and p450 cytochrome (p=0.001). Since p450 cytochrome participates in detoxification function, results indicate that glycaemia could affect detoxification. It will be important in future studies to study the implications of those results on the development of diabetes complications. The novelty of the study was to use PAS to find out if there was any correlation between spectroscopy variables and glycaemia. It is concluded that PRM applied to PAS is a suitable technology to study p450 cytochrome in diabetes.