Abstract
3-Cyanovinylcarbazole nucleoside (CNVK), which has an ultrafast photo-cross-linking ability to complementary RNA, was incorporated into the antisense oligonucleotide targeting green fluorescent protein (GFP) mRNA. We successfully demonstrated that the photoreactive antisense oligonucleotides effectively regulate GFP expression in a stable cell line expressing the GFP gene in a photo-responsive manner.
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