Abstract
DTIC or dacarbazine is a photosensitive drug which is analyzed by chromatography methods. In this study, chemometric methods were applied to resolve the UV–Vis absorbance spectra recorded during irradiation of DTIC by an artificial lighting source. The photo-degradation reaction was monitored at different pH media (between 2 and 12). Multivariate curve resolution-alternative least square was then employed to simultaneously analyze the absorbance data matrices obtained at various pH media. Factor analysis revealed the presence of five chemical species co-existing in the reaction media. Regarding the analysis of concentration profiles of the detected species at different pH values, three species were attributed to the different acid–base states of DTIC, while the remaining components were considered as acid–base states of the photoproduct. Eventually, the presence of major photoproduct (2-azahypoxanthine) was confirmed by the HPLC method.
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