Photo-Crosslinkable Double-Network Hyaluronic Acid Based Hydrogel Dressing

Abstract

Hyaluronic acid (HA)-based hydrogels are widely used in biomedical applications due to their excellent biocompatibility and enzymatic degradability. In this paper a photo-crosslinking double-network hyaluronic acid-based hydrogel dressing was proposed. Hyaluronic acid can be UV-crosslinked by modification with methacrylic anhydride (HA-MA) and disulfide-crosslinked by modification with 3,3'-dithiobis (propionylhydrazide) (DTP) (HA-SH). The mixings of these two materials at different ratios were produced. All the samples can be quickly gelled at 365 nm for 10 s. The rheological tests show that the storage modulus (G') of the double network (HA-SH/HA-MA) hydrogel is increased with the increase of HA-SH content. The HA-SH/HA-MA hydrogel has porous structure, high swelling ratio and Controlled degradation rate. In vitro degradation tests show that the ratio of HA-SH/HA-MA ratio was 9:1 (S9M1) in 100 U/ml hyaluronidase (Hase) degraded by 89.91±2.26% at 11d. The cytocompatibility of HA-SH/HA-MA hydrogels was proved by Live/Dead stainings and CCK-8 assays in the human dermis fibroblasts (HDF) cells test. All these results highlight the biological potential of the HA-SH/HA-MA hydrogels for DFU intervention.