Abstract
We quantitatively investigated the photo-bleaching of d-luciferin in aqueous solutions by UV-light irradiation at 325-, 365-, and 405-nm wavelengths. As a function of UV-light irradiation duration, we measured the numbers of undamaged d-luciferin molecules by quantitative bioluminescence experiments. Single-exponential fitting was performed to obtain the photo-bleaching rate per irradiation time, from which we extracted the photo-bleaching cross-sections σB defined as product ϕ×σA of the photo-absorption cross-section σA and photo-bleaching quantum yield ϕ of photo-excited d-luciferin. σB was about 0.8–4.5 × 10−20 cm2, while σA was about 1.3–6.3 × 10−17 cm2. Our findings showed that ϕ is 7 × 10−4 independently of UV-light wavelength.
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