Abstract
In cyanobacteria, the excess electrons produced through photosynthetic electron transport chain at light intensities, are re-oxidized by respiratory terminal oxidases (RTOs) present in the respiratory electron transport chain to minimize photo damage. By knocking out the genes responsible for the RTOs, the organism’s extracellular electron transfer (EET) ability towards the electrode could be further enhanced. In this study, the genes responsible for the RTOs in Synchococcus elongatus (S. elongatus) PCC7942 namely bd-type quinol oxidase, aa3-type cytochrome oxidase and cbb3-type cytochrome oxidase were knocked out individually and in combination to construct single and double mutants. PCR was carried out to confirm the successful construction of mutants. EET ability and photocurrent generation ability of the cyanobacteria in a photo bioelectrochemical cell were investigated through the ferricyanide reduction assay and conventional electrochemical experiments respectively.
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