Abstract
Photo-responsive caged compounds have high potency in the application concerning functional biological molecules as photo-switching device. Kinesin is a motor protein that moves along microtubule by the energy generated from ATP hydrolysis. The structure of conventional kinesin has been well studied and the key regions related to the function were clarfied. In the present study, the photo-regulation of the catalytic activity of mouse brain and C. elegans kinesins were investigated by treating with a caging reagent, 4,5-dimethoxy-2-nitrobenzyl bromide (DMNBB). The mouse brain kinesin mutants that have a single reactive cysteine at 96C were prepared and modified by DMNBB in the presence and absence of ATP. For the kinesin modified in the absence of ADP, the ATPase activity was increased by 300% within 10 minutes. In the presence of ADP, the change of the ATPase activity was slower than that in the absence of ATP. Upon UV irradiation, the ATPase activity of the kinesin modified by DMNBB recovered to the level before modification. Wild type of C. elegans unc-116 kinesin motor domain derived from C. elegans, which has a single reactive cysteine residue. Modification with DMNBB and photo-irradiation on the wild type of C.elegans kinesin unc-116 showed also significant reduction and restoration of activity. We have identified the amino acid residue of kinesin unc-116, which affects activity by the modification with DMNBB as Cys16.
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