Photic and non-photic effects on the daily activity pattern of Mongolian gerbils

Abstract

The paper analyses the daily activity pattern of Mongolian gerbils with and without access to a running wheel. To evaluate the synchronizing and the masking effects of light, experiments were performed under different photoperiods (L:D = 14:10 h and 10:14 h), and light and dark pulses were applied at different phases of the day–night cycle. In order to get a more direct estimate of the central pacemaker of the circadian system, the body temperature rhythm was investigated via implanted transmitters. Without access to a running wheel, the daily activity pattern was bimodal. One peak occurred in the first half of the light time, the other one around the light–dark transition. Also, the gerbils were more active during the light phase as compared to the dark phase. After unlocking the running wheel, the gerbils were active mainly during the dark time. The activity peak in the first half of the light phase remained, the second one shifted by a phase delay into the dark time. These results were found under both LD-regimens. Light during the night nearly completely suppressed running wheel activity, switching off the light during the day time induced wheel running. Whereas wheel running was clearly affected by light and dark pulses, the general activity was not. The body temperature rhythm also shows two peaks, with the second one being bigger and coinciding with the endogenous component of the circadian body temperature rhythm. It was found around light-off. After unlocking the running wheel, the maximum of the body temperature rhythm shifted to the night. This was not primarily a consequence of the changed activity pattern as shown by means of purification analysis. Removing the direct effects of motor activity led to a body temperature curve that could be described by a cosine function, and the delay shift was found also for the purified data, a better estimate of the endogenous circadian component. The wheel-associated increase in nocturnality is not only due to masking effects of wheel-running activity on the body temperature and activity rhythms. It also involves clock-related processes. Changes in the phase preference may serve as an adaptation mechanism to the changes in the animal's natural environment.