Phosphoserine and specific types of its coordination in copper(II) and adenosine nucleotides systems – Potentiometric and spectroscopic studies

Abstract

Ternary systems of copper(II) complexes with phosphoserine and adenosine 5'-monophosphate or adenosine 5'-diphosphate or adenosine 5'-triphosphate have been investigated. The studies have been performed in aqueous solution using the potentiometric method with computer analysis of the data, (13)C and (31)P nuclear magnetic resonance, visible and electron paramagnetic resonance spectroscopies. The overall stability constants of the complexes have been determined. Analysis of the equilibrium constants of the reaction have allowed determination of the effectiveness of the phosphate groups and donor atoms of heterocyclic rings in the process of complex formation. The potential reaction centres are the nitrogen atoms N(1) and N(7) and the phosphate group of the nucleotides as well as phosphate, carboxyl and amine groups from phosphorylated serine. Coordination sites of investigated ligands have been identified on the basis of the equilibrium constants analysis and spectroscopic studies.