Phosphorylation of nuclear matrix proteins in isolated regenerating rat liver nuclei

Abstract

The phosphorylation of nuclear matrix proteins from normal and regenerating rat liver nuclei was examined using an in vitro system of isolated nuclei and γ- 32P-ATP. Phosphorylation of the nuclear matrix proteins was 2–3 fold higher than that of the total nuclear proteins in normal nuclei. The level of phosphorylation of the matrix proteins was enhanced an additional three fold at a period in liver regeneration (12 hours) just preceding the onset of DNA synthesis.